intraperitoneal injection in humans

Kyriakou, C., Rabin, N., Pizzey, A., Nathwani, A. Epub 2010 Mar 3. . 2018 Jul;6(3):207-216. doi: 10.29252/beat-060304. Ko, I. K. et al. A relatively short needle (e.g., 0.5-inch or less) is suggested to help prevent puncture of the intestines or cecum. Another example is applying hammock catheter placement technique60. They are mitomycin C, cisplatin, carboplatin, oxaliplatin, irinotecan, 5-fluorouracil, gemcitabine, paclitaxel, docetaxel, doxorubicin, premetrexed, and melphalan. One article reviews the injection of sodium pentobarbital to euthanize rodents intraperitoneally. MSCs induced more Ki-67 positive cells in the lower part of the crypt than the DSS control. & Yong, K. Factors that influence short-term homing of human bone marrow-derived mesenchymal stem cells in a xenogeneic animal model. prepared Figure 4. Neutrophil infiltration was monitored by measuring MPO activity67. Mouse insulin gradually decreased and was no more detectable 13 weeks after STZ injection. Cochrane Database Syst Rev. Importantly, we identified different migration fates of MSCs and traced their ultimate location of three administration ways for the first time. In addition, fewer female mice achieved surgical anesthesia, suggesting a narrow therapeutic window for intraperitoneal KX in female mice. While the DiR labeled cells showed very different cell distribution. Curr Opin Gastroenterol 30, 8496, doi: 10.1097/MOG.0000000000000031 (2014). In IV group, most of MSCs were trapped in the lung, liver and spleen, while the cell that immigrated to the colon was not too much. Shamsi M, Sedaghatkish A, Dejam M, Saghafian M, Mohammadi M, Sanati-Nezhad A. Dou, S., Smith, M., Wang, Y., Rusckowski, M. & Liu, G. Intraperitoneal injection is not always a suitable alternative to intravenous injection for radiotherapy. Stem cells (Dayton, Ohio) 24, 386398, doi: 10.1634/stemcells.2005-0008 (2006). Comparative analysis of chemokine receptors expression in mesenchymal stem cells derived from human bone marrow and adipose tissue. After intraperitoneal injections, 30% of the female mice died, compared with 3% of the male. Blood 113, 4657, doi: 10.1182/blood-2008-04-154138 (2009). Stem cell transplantation showed promising results in IBD management. Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing. Two early reports aroused the global interests of stem cell transplantation in IBD management. In mouse, the intraperitoneal and subcutaneous LD 50 values are 138 and 650 mg kg 1, respectively. The good cell-cell cross talk between MSCs and immune cells may be another contributor for better therapeutic effects. Unable to load your collection due to an error, Unable to load your delegates due to an error. A simple dengue vaccine evaluation system was established using a model of dengue type 2 virus (DENV2) infection in immunocompetent mice. Slow intravenous injections are typically dosed over 3-10 minutes. Therefore, the MSCs delivery should be tailored to the lesion kind and customized to the mechanism of action of MSCs. Histological evaluation was completed in a double-blind fashion by two independent pathologist. IP, intraperitoneal injection; IV, intravenous injection; AI anal injection; n = 6-8 for treatment group, n = 3 for DSS control. Adv Exp Med Biol 371A, 6165 (1995). Whole marrow cells are plated in the culture dish and non-adherent cells were removed during passage. Proliferation index was defined as percent of Ki-67 positive nuclei within the random visual scope. Methods of euthanasia likely to elicit distress vocalizations or pheromones that other animals could hear or smell should be performed in another location, if transportation distress can be minimized. There are increasing evidences showed that MSCs mediate their immunomodulatory effects through the induction of regulatory T cells (Treg cells)14. ISSN 2045-2322 (online). Effects of different mesenchymal stromal cell sources and delivery routes in experimental emphysema. Intraperitoneal injection carries the risk of causing internal injury or of misplaced injection into a blood vessel or an organ, particularly the large thin-walled cecum or urinary bladder. Infiltration (I): 0, no infiltrate; 1, infiltrate around crypt basis; 2, infiltrate reaching to lamina muscularis mucosa layer; 3, extensive infiltration reaching the muscularis mucosa with abundant edema; 4, infiltration of the submucosa layer. (B) KaplanMeier analysis of three different MSCs delivery ways. In COX-2 blocking control experiments, mice received FA-NPs (0.5 mg/kg) 1 h after an intraperitoneal injection of celecoxib (10 mg/kg) to block binding of FA to the COX-2 active site. Cells, tissues, organs 169, 1220 (2001). For some severe cases, IBD may lead to the life-threaten complications5. query serum metabolite genome-wide association study (GWAS) data to identify an association between choline metabolism and feline leukemia virus subgroup C cellular receptor 1 (FLVCR1). A study was conducted to determine the best route of administration to transplant mesenchymal stem cells for colitis. Respir Res 15, 118, doi: 10.1186/s12931-014-0118-x (2014). Role for interferon-gamma in the immunomodulatory activity of human bone marrow mesenchymal stem cells. However, for IV injection, we and others found most mice will lead increased mortality because of potential pulmonary cell embolus when the cell dose increase to 110722. Supplies: The first major problem with IV route is the pulmonary first-pass effects, which cause significant entrapment of cells15. However, one recent published paper showed inconsistent results with ours, in which IV injection was identified better in comparison with intraperitoneal injection31. Gastroenterology 136, 978989, doi: 10.1053/j.gastro.2008.11.041 (2009). gynecological and gastrointestinal cancers and has shown very promising results. injections. PubMed mRNA expression of inflammatory mediators such as TNF-, IL-6, IL-1b, IL-10 and IFN- in the colon were measured by real time PCR. Horses and other large animals: 1 mL per 10 lb of body weight, to a maximum . Rep. 6, 30696; doi: 10.1038/srep30696 (2016). [2], There has been some debate on whether intraperitoneal injections are the best route of administration for experimental animal studies. 2021 Dec;12(1):8635-8649. doi: 10.1080/21655979.2021.1988364. Isolated allogeneic bone marrow-derived mesenchymal cells engraft and stimulate growth in children with osteogenesis imperfecta: Implications for cell therapy of bone. A human study showed that after injection of HDL labeled with a CE tracer, most of the tracer that was ultimately found in the bile arrived there after transfer to apoB-containing lipoproteins, . Epidemiology and natural history of inflammatory bowel diseases. In order to compare the difference of MSCs colonization among the three delivery routes, a nontoxic NIR tracer DiR was introduced to label MSCs. found MSCs improved cardiac function by a remote way46. Exposure to BHT through inhalation, dermal, and oral routes is harmful. To determined cytokine in colon mucosa, protein extracts were isolated by homogenization of colonic segments (50mg tissue/mL) in 50mmol/L Tris-HCl, pH 7.4, with 0.5mmol/L dithiothreitol and 10 g/mL of a cocktail of proteinase inhibitors (Sigma). C57BL/6 mice were brought from (68 weeks; SLACCAS, Shanghai, China), GFP transgenic mice ware brought from (The institute of Laboratory Animal Science, Chinese Academy of Medical Sciences & Peking Union Medical College, China). Study showed that genetically modified MSCs to increase CXCR-4 expression will lead to an increase of MSCs migration to intestinal with radiation enteritis and then resulted in disease improvement24. Collagen deposition is another indicator for wound healing, however, extensive collagen deposition may hamper the gut reconstitution14. Stem cells and development 18, 683692, doi: 10.1089/scd.2008.0253 (2009). ADS Lopez-Cubero, S. O., Sullivan, K. M. & McDonald, G. B. Best way for mesenchymal stem cells transplantation for colitis. Future Oncol 6, 16251641, doi: 10.2217/fon.10.100 (2010). How to cite this article: Wang, M. et al. Your peritoneal (payr-ih-toh-NEE-ul) space is the area between the muscles and organs in your abdomen (belly). : 1950) 180, 15981608 (2008). Nature biotechnology, doi: 10.1038/nbt.2816 (2014). After centrifuge, the cells were certificated and transferred to MSC expansion culture medium consisting of a-minimum essential medium (a-MEM; Gibco, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA) and 100g/mL penicillin/streptomycin. Vilquin, J. T. & Rosset, P. Mesenchymal stem cells in bone and cartilage repair: current status. This review discusses the usefulness of IP drug therapy and the factors influencing it, as well as strategies to increase the efficacy, and conclude that IP route is an alternate route to the more conventional drug delivery routes, and can be successfully used when the target is within the peritoneal cavity or adjacent tissue. MPO activity per gram of wet tissue was calculated as: MPO activity (U/g wet tissue)=(A450) (13.5)/tissue weight (g). Three days after MSCs transplatation, the mice were sacrificed for histological evaluation (H&E and phosphomolibidic acid-picrosirius red staining). Cancer Biother Radiopharm 28, 335342, doi: 10.1089/cbr.2012.1351 (2013). These cells were confirmed as bone marrow MSCs using flow-cytometric analysis and differential identification (Supplementary Figures S1 and S2). Intraperitoneal injections may also allow too-rapid entry of bacteria or LPS into the blood. 7B). drug availability and human abuse potential; 10. compatibility with species, age, and health status; 11. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. Briefly, colonic segments were homogenized at 50mg/mL in phosphate buffer (50mM, pH 6.0) with 0.5% hexadecyltrimethylammonium bromide. Since that time, the literatures using stem cells for IBD have expanded. Stem cell reviews 10, 127144, doi: 10.1007/s12015-013-9468-x (2014). Then sections were washed and blocked with normal nonimmunone goat serum for 30min. One study utilized IP injections to study pain in the abdomen after a hysterectomy when administering anesthetic continuously vs patient-controlled. Intraperitoneal injection or IP injection is the injection of a substance into the peritoneum (body cavity). Article In the guinea pig, the oral LD 50 value is 10 700 mg kg 1. The IP injection significantly inhibited collagen deposition compared with DSS control (P=0.0008) and IV group (P=0.0139). IBD can be painful and persistent for life, which might decrease the quality of life. and S.J. Histopathological comparison of colitis after 3 days MSCs delivery. Kalchenko, V. et al. Fluorescent intensity was quantified and processed using the Living Image Software (Version 4.2; Caliper Life Science). designed the research. Samples were frozen and thawed 3 times, centrifuged at 30,000g for 20minutes. The serum concentration of TNFinduced protein 6 (TSG-6) was measured in DSS colitic mice at 48 and 72hours after different routes of MSCs administrations. By systemic evaluation of epithelium and lymphocyte infiltration, the IP injection group showed the relatively lower severity score compared with AI group (P=0.034) and IV (P=0.002), respectively. 5B). 2014ZX09508002). Furthermore, the corresponding mesentery lymph nodes (MLNs) also showed relatively high MSCs existence (Fig. Thus there is a strong impetus to seek more effective approaches for disease management. Makhija, S. et al. Second concerning is about the cell dose. Our findings showed that the GFP+ MSCs were migrated to the inflamed colon, even passed through the whole intestinal wall and reached the luminal side. Wang, N. et al. [citation needed], In humans, the method is widely used to administer chemotherapy drugs to treat some cancers, particularly ovarian cancer. 8600 Rockville Pike Burisch, J. et al. However, no significant TSG-6 difference was found between AI and IV groups. 81371615), the Stake Key Laboratory Open Program of China (No. Some of the examples depict situations where IP injections are not ideal, while others prove the advantageous uses if this delivery method. Available at: http://ctep.cancer.gov/highlights/20060105_ovarian.htm (Accessed: 7th April 2016) (2006). Very few reports pay attention to factors applied in the clinic, such as injection or exposure routes, which has highly impact on stem cell therapeutic efficiency for IBD12. Stem cells international 2013, 732742, doi: 10.1155/2013/732742 (2013). Muller-Ehmsen, J. In various preclinical models, saroglitazar has been shown to prevent & reverse symptoms of NASH. [2] Killing the rodent through an intraperitoneal route was originally recommended over other routes such as inhalants because it was thought to be more efficient and ethical. We notice that the mean density of collagen was lowest in IP group, however, IP group did not exhibit less collagen deposition than the AI group, probably due to the scatter points (Fig. MSCs has been found to directly differentiate into end-stage cells to augment bone damages and repair cartilage defects38,39. An example of when intraperitoneal injections are not ideal is given in a study where the best route of administration was determined for cancer biotherapy. It was concluded that there is evidence that IP may not be the best method of euthenasia due to possibilities of missinjection. The histological score was defined as the sum of the two parameters (total score=E+I). These mice were then randomized into 3 groups receiving different MSCs giving therapies. IHC was performed according to the standard procedures. ", "Intraperitoneal injection (IP), Intravenous injection (IV) or anal injection (AI)? The fluorescence imaging disclosed higher intensity of engrafted cells in inflamed colon and the corresponding mesentery lymph nodes (MLNs) in IP and AI groups than the IV group. On the contrary, the anti-inflammatory IL-10 level were substantially elevated in IP group compared with the IV group and DSS control, but the inter-group difference between IP and AI group, AI and IV group were not identified. Atreya, R. & Neurath, M. F. Chemokines in inflammatory bowel diseases. (A) MSCs were labeled with DiR dye and given by three different ways. Our results indicated that the intraperitoneal injection is the best delivery way for MSCs, which showed better mucosa recovery and higher cell engraftment at inflamed colon. Gastroenterology 132, 944954, doi: 10.1053/j.gastro.2006.12.029 (2007). and H.H. Such huge need of MSCs poses a cell source issue, which is a continuing technically and operationally challenge, since most of human ongoing clinical trials use significant less cell dose12. Insert the needle at a shallow angle through the . Google Scholar. Interaperitoneal Administration of lpha-Tocopherol Loaded Nanoparticles Improves Ischemia-Reperfusion Injury in Rat Ovaries Torsion and Detorsion Model. Brown, S. L. et al. The bilateral femurs and tibias were aseptically excised, stripped of connective tissues and then stored in PBS. The safety and scientific validity of this study is the responsibility of the study sponsor and investigators. Am J Gastroenterol 105, 289297, doi: 10.1038/ajg.2009.579 (2010). MSCs have been well characterized regarding their ability to produce a range of cell cytokines, which have a profound effect on modulating the immune system. Mice were maintained in a light/temperature-controlled room and allowed to freely access to chow diet and water. Clinical and experimental immunology 116, 238245 (1999). Two patients with long-standing ulcerative colitis, psoriasis and leukemia underwent allogeneic stem cell transplantation and all the three disorders were in clinical remission for 4 years after transplantation11. Google Scholar. Castelo-Branco, M. T. et al. In their study, PKH26 (a yellow-orange fluorescent dye) labeled PCs were injected intraperitoneally into healthy rat. The labeled cells were twice with warm fresh medium at 1500rpm for 5min to ensure complete removal of any unbound dye. 2021. Just mentioned above, immune cells and MSCs interaction in aggregates promotes TSG-6 secretion and contributed to the colitis recovery. Meanwhile, GFP+ MSCs (collected from transgenic mice) were used to determine MSCs specific location. IP injected human MSC rapidly formed aggregates with mouse macrophages and B220+ lymphocytes and attached to the walls of the peritoneal cavity. Rodent (large / mouse) oral (intragastric), intravascular administration (tail vein injection, intraperitoneal injection, intramuscular injection and subcutaneous injection) Intragastric administration and oral taking of non-rodent (dog / monkey) solution and intravenous injection of extremities. Half of the volume of your D-luciferin substrate can be injected on one side of the animal and the other half of the volume on the opposite side. The results set a precedent of how other drugs may be delivered in this way to treat other similar medical issues after further research. Clinical Announcement: Intraperitoneal chemo-therapy for ovarian cancer. It is estimated that as many as 1.4million Americans and 2.4million Europeans are suffering from these diseases1,2. Free DiR and healthy mice (without colitis) were served as control. Mice were made diabetic by intraperitoneal injection of streptozotocin, which led to a 2- to 2.5-fold increase in plasma glucose. Although controversial, intraperitoneal use in ovarian cancer has been recommended as a standard of care. Cell stem cell 9, 1115, doi: 10.1016/j.stem.2011.06.008 (2011). GFP+ cells were detected at the inflamed colon. Human. Note where each injection is made and subsequent injections should be made in slightly different spot than the previous injection. Adhesions are a leading cause of secondary infertility in women, accounting for 15% to 20% of cases. government site. At 72h, the IP groups showed significant higher level of TSG-6 than AI groups and DSS control (P=0.023 and P=0.005, respectively). In addition, even MSCs could formed aggregates in those harsh conditions, there still be a highly risk of self-removal. The Scheme (Fig. PLGA Nanoparticles for the Intraperitoneal Administration of CBD in the Treatment of Ovarian Cancer: In Vitro and In Ovo Assessment. 17 The influence of the sex of the animal on anesthetic agents remains poorly researched and understood.
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